Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming pools

作者：Staggemeier Rodrigo^*; Arantes Thalita; Caumo Karin S; Rott Marilise B; Spilki Fernando R

来源：ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS, 2016, 88(1): 635-641.

DOI：10.1590/0001-3765201620150151

• Acanthamoeba
• free-living amoebae
• human adenovirus
• qPCR
• swimming pools

Acanthamoeba is the most common free-living environmental amoeba, it may serve as an important vehicle for various microorganisms living in the same environment, such as viruses, being pathogenic to humans. This study aimed to detect and quantify human adenoviruses (HAdV) in Acanthamoebas isolated from water samples collected from swimming pools in the city of Porto Alegre, Southern Brazil. Free-living amoebae of the genus Acanthamoeba were isolated from water samples, and isolates (n=16) were used to investigate the occurrence of HAdVs. HAdV detection was performed by quantitative real-time polymerase chain reaction (qPCR). HAdVs were detected in 62.5% (10/16) of Acanthamoeba isolates, ranging from 3.24x10(3) to 5.14x10(5) DNA copies per milliliter of isolate. HAdV viral loads found in this study are not negligible, especially because HAdV infections are associated with several human diseases, including gastroenteritis, respiratory distress, and ocular diseases. These findings reinforce the concept that Acanthamoeba may act as a reservoir and promote HAdV transmission through water.

• 出版日期2016-5