Absorption mechanism of whey-protein-delivered curcumin using Caco-2 cell monolayers

作者：Li Ming; Cui Jie; Ngadi Michael O^*; Ma Ying

来源：Food Chemistry, 2015, 180: 48-54.

DOI：10.1016/j.foodchem.2015.01.132

• Curcumin
• beta-Lactoglobulin
• Nanoemulsion
• Permeability

Curcumin (CCM) is a bioactive polyphenolic compound that suffers a low bioavailability because of its low water solubility. In this work p-lactoglobulin (beta-Lg) and nanoemulsion were used as carriers to deliver curcumin. The pH stability of beta-Lg-CCM was investigated. The digestion of beta-Lg-CCM and the nanoemulsion was studied using an in vitro gastrointestinal model. The effect of different carriers on the permeability of curcumin was assessed using the Caco-2 cell monolayer model. The results revealed that the water solubility and the pH stability of curcumin significantly increased by binding with beta-Lg. In SDS-PAGE experiments the beta-Lg-CCM complex and nanoemulsion were found to be resistant to pepsin digestion but sensitive to trypsin. In the permeability experiment it was shown that the digested nanoemulsion and beta-Lg-CCM improved significantly the permeation rate of curcumin.

• 出版日期2015-8-1
• 单位哈尔滨工业大学; 通化师范学院