Allodiplogaster josephi n. sp and A-seani n. sp (Nematoda: Diplogastridae), associates of soil-dwelling bees in the eastern USA

作者：Kanzaki Natsumi^*; Giblin Davis Robin M; Ragsdale Erik J

来源：Nematology, 2015, 17(7): 831-863.

DOI：10.1163/15685411-00002908

• Andrena alleghaniensis
• Andrenidae
• Colletes thoracicus
• Colletidae
• description
• molecular
• morphology
• morphometrics
• new species
• phoresy
• phylogeny
• taxonomy

Two commensal associates of bees, Allodiplogaster josephi n. sp. from the Dufour's gland of a cellophane bee (Colletes thoracicus) from Maryland, USA, and A. seani n. sp. from the abdominal glands of an andrenid bee (Andrena alleghaniensis) from New York, USA, are described and illustrated. Both species were collected as dauers from their respective hosts and cultured on bacteria on tryptic soy broth (TSB) or NGM agar. Allodiplogaster josephi n. sp. and A. seani n. sp. are morphologically closer to each other than to other species of Allodiplogaster, which was recently revised to include 37 valid species. However, the two new species are distinguished by reproductive isolation, shape of the spicule manubrium, host associations and molecular characters, the latter in sequences of the near-full length small subunit (SSU) rRNA gene, D2-D3 expansion segments of the large subunit (LSU) rRNA gene and partial mitochondrial COI. Morphological characterisation was supplemented by scanning electron microscopy (SEM), which revealed furcation of both v5 and v6 male genital papillae, consistent with previous reports for species of the henrichae group of Allodiplogaster.

• 出版日期2015