Adaptation of Pseudomonas aeruginosa to a pulsed light-induced stress

作者：Massier S; Rince A; Maillot O; Feuilloley M G J; Orange N; Chevalier S^*

来源：Journal of Applied Microbiology, 2012, 112(3): 502-511.

DOI：10.1111/j.1365-2672.2011.05224.x

• adaptation
• proteome
• Pseudomonas aeruginosa
• pulsed light technology
• pulsed light-induced stress

Aims: Pulsed light (PL) technology is an efficient surface decontamination process. Used in low transmitted energy conditions, PL induces a stress that can be perceived by bacteria. The effect of such a PL stress was investigated on the highly environmental adaptable germ Pseudomonas aeruginosa PAO1. %26lt;br%26gt;Methods and Results: Pulses of transmitted energy (fluence) reaching 1.8 J cm(-2) can kill 10(9) bacteria. Application of a lower sublethal PL dose allowed the bacteria to resist and survive more efficiently to a subsequent dose of PL. This sublethal dose was not increasing the mutation frequency of Ps. aeruginosa, but altered the abundance of 15 proteins as revealed by a global proteome analysis, including stress-induced proteins, phage-related proteins, energy and carbon metabolisms, cell motility, and transcription and translation regulators. %26lt;br%26gt;Conclusions: A response to a low-energy PL dose takes place in Ps. aeruginosa, reducing the energy conversion systems, while increasing transcription and translation processes to produce proteins involved in chaperone mechanisms and phage-related proteins, probably to protect the bacterium against a new PL-induced stress. %26lt;br%26gt;Significance and Impact of the Study: Taken together, these results suggest that a low-energy PL dose is sufficient to provoke adaptation of Ps. aeruginosa, leading to enhancing its resistance to a subsequent lethal treatment.

• 出版日期2012-3