Acceleration of azo dye decolorization by using quinone reductase activity of azoreductase and quinone redox mediator

作者：Liu Guangfei; Zhou Jiti; Wang Jing; Zhou Mi; Lu Hong; Jin Ruofei^*

来源：Bioresource Technology, 2009, 100(11): 2791-2795.

DOI：10.1016/j.biortech.2008.12.040

• Azo dye
• Azoreductase
• Decolorization
• Quinone reductase
• Redox mediator

This study demonstrated the effective application of intracellular azoreductase in mediated decolorization of azo dyes. Using the quinone reductase activity of overexpressed azoreductase AZR and quinone redox mediators, the decolorization performance of the recombinant strain Escherichia coli YB was significantly enhanced. in the presence of 0.2 mM lawsone, 75% acid red 27 (1 mM) was decolorized by E. coli YB in only 2 h, which was the highest bacterial decolorization rate ever reported. Compared to lawsone, menadione was a less effective redox mediator. Glucose was found to be the best carbon source for mediated decolorization by E. coli YB. The recombinant strain could complete four rounds of mediated decolorization repeatedly in 12 h. In addition, a 10-min pre-incubation of E. coli JM 109 and activated sludge with 2-methylhydroquinone resulted in great improvement of mediated decolorization performance, which may be applied in practical treatment.

• 出版日期2009-6
• 单位大连理工大学