Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania

作者：Mugasa Claire M; Deborggraeve Stijn; Schoone Gerard J; Laurent Thierry; Leeflang Mariska M; Ekangu Rosine A; El Safi Sayda; Saad Al farazdag A; Basiye Frank L; De Doncker Simone; Lubega George W; Kager Piet A; Buscher Philippe; Schallig Henk D F H^*

来源：Tropical Medicine and International Health, 2010, 15(7): 800-805.

DOI：10.1111/0365-3156.2010.02547.x

• leishmaniasis
• trypanosomiais
• diagnostics
• nucleic acid sequence-based amplification
• polymerase chain reaction
• oligochromatography

OBJECTIVE To evaluate the repeatability and reproducibility of four simplified molecular assays for the diagnosis of Trypanosoma brucei spp. or Leishmania ssp. in a multicentre ring trial with seven participating laboratories.
METHODS The tests are based on PCR or NASBA amplification of the parasites nucleic acids followed by rapid read-out by oligochromatographic dipstick (PCR-OC and NASBA-OC).
RESULTS On purified nucleic acid specimens, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 91.7% and 95.5%; Tryp-NASBA-OC, 95.8% and 100%; Leish-PCR-OC, 95.9% and 98.1%; Leish-NASBA-OC, 92.3% and 98.2%. On blood specimens spiked with parasites, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 78.4% and 86.6%; Tryp-NASBA-OC, 81.5% and 89.0%; Leish-PCR-OC, 87.1% and 91.7%; Leish-NASBA-OC, 74.8% and 86.2%.
CONCLUSION As repeatability and reproducibility of the tests were satisfactory, further phase IT and III evaluations in clinical and population specimens from disease endemic countries are justified.

• 出版日期2010-7