Histone deacetylase inhibitors block nuclear factor-kappa B-dependent transcription by interfering with RNA polymerase II recruitment

作者：Furumai Ryohei; Ito Akihiro; Ogawa Kenji; Maeda Satoko; Saito Akiko; Nishino Norikazu; Horinouchi Sueharu; Yoshida Minoru^*

来源：Cancer Science, 2011, 102(5): 1081-1087.

DOI：10.1111/j.1349-7006.2011.01904.x

Histone deacetylase inhibitors (HDACi) have been shown to exhibit anti-inflammatory activity, but their mechanism of action is poorly understood. Trichostatin A (TSA) and the cyclic tetrapeptide class inhibitor Ky-2 inhibit both lipopolysaccharide-induced tumor necrosis factor-alpha (TNF-alpha) production in rats and TNF-alpha-induced expression of inflammatory genes in HeLa cells. We assessed the molecular mechanism underlying TSA-induced anti-inflammatory activity by genetically dissecting activation of the nuclear factor-kappa B (NF-kappa B) pathway following stimulation with TNF-alpha. Trichostatin A did not inhibit degradation of I kappa B alpha, nuclear translocation and DNA binding of NF-kappa B; also, the drug did not affect transient expression from exogenous kappa B-reporter plasmids. However, endogenous expression of inflammatory cytokines such as interleukin-8 (IL-8) was greatly reduced, even in the absence of de novo protein synthesis, suggesting that HDACi directly inhibits NF-kappa B-induced transcription. Indeed, chromatin immunoprecipitation (ChIP) analysis showed that events related to transcriptional activation of the IL-8 gene region in response to TNF-alpha, including recruitment of RNA polymerase II (Pol II), were compromised in the presence of TSA. These data indicate that HDAC activity is required for the efficient initiation and/or elongation of inflammatory gene transcription mediated by NF-kappa B. (Cancer Sci 2011; 102: 1081-1087).

• 出版日期2011-5