ARP101, a selective MMP-2 inhibitor, induces autophagy-associated cell death in cancer cells

作者：Jo Yoon Kyung; Park So Jung; Shin Ji Hyun; Kim Yunha; Hwang Jung Jin; Cho Dong Hyung^*; Kim Jin Cheon

来源：Biochemical and Biophysical Research Communications, 2011, 404(4): 1039-1043.

DOI：10.1016/j.bbrc.2010.12.106

• Autophagy
• ATG5
• Cell death
• ARP101
• Cancer cell

Autophagy is a catabolic cellular process involving self-digestion and turnover of macromolecules and entire organelles. Autophagy is primarily a protective process in response to cellular stress, but it can be associated with cell death. Genetic evidence also supports autophagy function as a tumor suppressor mechanism. To identify specific regulators to autophagy, we screened the Lopac 1280 and the Prestwick chemical libraries using a cell-based screening system with autophagy marker (green fluorescence protein conjugated LC3 protein (GFP-LC3)). We identified ARP101, a selective matrix metalloproteinase-2 (MMP-2) inhibitor as one of the most potent inducer of autophagy. ARP101 treatment was highly effective in inducing the formation of autophagosome and conversion of LC3I into LC3II. Moreover, ARP101-induced autophagy was completely blocked in mouse embryo fibroblasts that lacked autophagy related gene 5 (ATG5(-/-) MEF). Interestingly, cell death induced by ARP101 was not inhibited by zVAD, a pan caspase inhibitor, whereas, it was efficiently suppressed by addition of 3-methyladenine, an autophagy inhibitor. These results suggest that the selective MMP-2 inhibitor, ARP101, induces autophagy and autophagy-associated cell death.

• 出版日期2011-1-28