An Enhanced Process for the Production of a Highly Purified Extracellular Lipase in the Non-conventional Yeast Yarrowia lipolytica

作者：Turki Saoussen; Ayed Atef; Chalghoumi Nejib; Weekers Frederic; Thonart Philippe; Kallel Hela^*

来源：Applied Biochemistry and Biotechnology, 2010, 160(5): 1371-1385.

DOI：10.1007/s12010-009-8599-7

• Yarrowia lipolytica
• Lipase
• Fed-batch culture
• Bioreactor
• Purification

Yarrowia lipolytica LgX64.81 is a non-genetically modified mutant that was previously identified as a promising microorganism for extracellular lipase production. In this work, the development of a fed-batch process for the production of this enzyme in this strain was described. A lipolytic activity of 2,145 U/mL was obtained after 32 h of batch culture in a defined medium supplemented with 10 g/L of tryptone, an enhancer of lipase expression. To maximize the volumetric productivity, two different fed-batch strategies had been investigated. In comparison to batch process, the intermittent fed-batch strategy had not improved the volumetric lipase productivity. In contrast, the stepwise feeding strategy combined with uncoupled cell growth and lipase production phases resulted in a 2-fold increase in the volumetric lipase productivity, namely, the lipase activity reached 10,000 U/mL after 80 h of culture. Furthermore, this lipase was purified to homogeneity by anion exchange chromatography on MonoQ resin followed by gel filtration on Sephacryl S-100. This process resulted in an overall yield of 72% and a 3.5-fold increase of the specific lipase activity. The developed process offers a great potential for an economic production of Lip2 at large scale in Y. lipolytica LgX64.81.

• 出版日期2010-3