Molecular cloning and characterization of a class II ADP ribosylation factor from the shrimp Marsupenaeus japonicus

作者：Zhang Mingchang; Ma Jinyou; Lei Kaiyu; Xu Xun^*

来源：Fish & Shellfish Immunology, 2010, 28(1): 128-133.

DOI：10.1016/j.fsi.2009.10.008

• Shrimp
• WSSV
• ADP ribosylation factor
• Defense

In shrimp, small GTPases in the Ras superfamily can regulate hemolytic phagocytosis against WSSV infection. However, the ADP ribosylation factors (Arfs), also belonging to the regulatory GTP-bincling proteins and playing a central role in membrane trafficking, have not been reported yet in shrimp and their relationship with WSSV infection is completely unknown to date. Here, a novel class II Arf (designated as MjArf4) was cloned and characterized from the shrimp Marsupenaeus joponicus. Like other Arfs, MjArf4 contains an N-terminal myristoylated site, a p loop, switch regions, as well as an interswitch region. In High Five cells, when MjArf4 was in its GDP-bound form, it dispersed into the whole cell, whereas in the GTP-bound form it promoted formation of a punctuate Golgi-like structure, indicating that the MjArf4 distribution was dependent on its GDP/GTP binding. After challenge with WSSV, the mRNA level of MjArf4 was up-regulated significantly as WSSV propagated. Thus, a member of the Arf family was characterized for the first time in shrimp and found to be involved in WSSV infection.

• 出版日期2010-1
• 单位河南科技学院; 国家海洋局第三海洋研究所; 厦门大学