Aquaporin-1 Expression in Proliferative Vitreoretinopathy and in Epiretinal Membranes

作者：Motulsky Elie^*; Salik Dany; Janssens Xavier; Pion Bart; Dufrane Rebecca; Chaput Florence; Bolaky Nargis; Gregoire Franoise; Caspers Laure; Perret Jason; Willermain Franois; Delporte Christine

来源：The Scientific World Journal, 2014, 2014: 876208.

DOI：10.1155/2014/876208

Purpose. Aquaporin-1 (AQP1) is involved in cell migration and proliferation; therefore, the purpose of the studywas to investigate its expression in proliferative vitreoretinopathy (PVR) and epiretinal membranes (ERM). Methods. 19membranes fromPVR and ERM were collected following eye surgery. AQP1mRNAand protein expressionswere determined by RT-qPCR and immunofluorescence in the membranes from PVR and ERM. Results. AQP1 mRNA and protein were expressed in both PVR and ERM as shown by RT-qPCR and immunofluorescence. AQP1 protein expression was heterogeneous among and between PVR and ERM and colocalized with alpha-smooth muscle actin (alpha SMA) and with glial fibrillary acidic protein (GFAP). There were a higher percentage of cells coexpressing AQP1 and alpha SMA than AQP1 and GFAP. GFAP and alpha SMA did not colocalize. Conclusion. Our data show for the first time AQP1 expression in both PVR and ERM. AQP1 is expressed mostly by the alpha SMA-positive cells, presumably myofibroblasts, but also by GFAP-positive cells, assumed to be glial cells. These original findings warrant further functional investigations aiming at studying the potential role of AQP1 in cell migration and proliferation occurring during the development of PVR and ERM.

• 出版日期2014