Downstream Recovery and Purification of a Bioemulsifier from Saccharomyces cerevisiae 2031

作者：Alcantara Virgie A^*; Pajares Irene G; Simbahan Jessica F; Edding Sherida N

来源：The Philippine Agricultural Scientist, 2013, 96(4): 349-358.

• emulsion
• emulsification activity
• emulsification index
• mannoprotein
• Saccharomyces cerevisiae 2031

Bioemulsifiers are high-molecular-weight biosurfactants that are able to stabilize oil-in-water emulsion. In this study, the bioemulsifier, a mannoprotein from Saccharomyces cerevisiae 2031, was purified using three protein precipitation methods and Sephadex G-75 gel filtration chromatography. The bioemulsifier consisted of 77% carbohydrate and 23% protein with a molecular weight of approximate to 88 kDa. Emulsification activity of the mannoprotein, as measured by emulsification index (E-24) was 69%. Emulsification activity of the crude bioemulsifier was significantly higher compared with that of the purified bioemulsifier. The latter formed stable emulsions with a variety of commercial oils, hydrocarbons, organic solvents and waste cooking oil. Emulsification activity was generally unaltered by extreme changes in pH, temperature and salinity. However, the bioemulsifier was found to be pH-sensitive. The study revealed important biochemical properties of the bioemulsifier from S. cerevisiae 2031. These properties could be exploited for possible applications in the food, cosmetics, detergent and pharmaceutical industries, including bioremediation of oil-contaminated and hydrocarbon-contaminated wastes.

• 出版日期2013-12