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摘要
In order to test the chromosome doubling effect of two antimitotic chemicals %26quot;trifluralin%26quot; and %26quot;acenaphthene%26quot;, two experiments were conducted separately, using androgenetic haploid plantlets with 3 to 5 leaves of tobacco %26quot;Nicotiana tabacum cv. Saribaglar%26quot;. Seed settings were considered in distinguishing dihaploids. %26lt;br%26gt;In the trifluralin experiment, plantlets were treated in flasks containing liquid Nitsch medium and 0, 10, 30 and 50 mu M trifluralin for 24, 48 and 72 h on a 150 rpm shaker. Fifty plantlets were used per treatment and experimental design was split-plotted with three replications. The highest chromosome doubling was recorded for 24 h treatment with 10 mu M concentration. %26lt;br%26gt;Acenaphthene experiment was carried out with 3 replications of monofactorial randomized plots. About 100 mg crystals were hanged into small sterilized cheesecloth bags into culture vessels (6x7cm) containing 5 haploid plantlets, for 48, 72 and 96 h. Fifty plantlets per treatment were used, and treatment effects were not significant, although average doubling ratios increased from 3.2 to 10.4% with increasing durations.
- 出版日期2014
