Differentiation of sow and mouse ovarian granulosa cells exposed to zearalenone in vitro using RNA-seq gene expression

作者：Zhang, Guo-Liang; Song, Jun-Lin; Zhou, Yi; Zhang, Rui-Qian; Cheng, Shun-Feng; Sun, Xiao-Feng; Qin, Guo-Qing; Shen, Wei; Li, Lan^*

来源：Toxicology and Applied Pharmacology, 2018, 350: 78-90.

DOI：10.1016/j.taap.2018.05.003

• ZEA
• Porcine
• Mouse
• Granulosa cells
• Gene expression
• RNA-seq

Zearalenone (ZEA), a natural contaminant found in feed, has been shown to have a negative impact on domestic animal reproduction, particularly in pigs. There are species-specific differences in the ZEA-induced toxicity pattern. Here, we investigated the different biological effects of ZEA exposure on porcine and mouse granulosa cells, using RNA-seq analysis. We treated murine and porcine granulosa cells with 10 mu M and 30 mu M ZEA during 72 h of culturing, in vitro. The results showed that 10 mu M ZEA exposure significantly altered mitosis associated genes in porcine granulosa cells, while the same treatment significantly altered the steroidogenesis associated genes in mouse granulosa cells. Exposure to 30 mu M ZEA resulted in significantly up-regulated expression of inflammatory related genes in porcine granulosa cells as well as the cancer related genes in mouse granulosa cells. Similarly, 30 mu M ZEA exposure significantly decreased the expression of tumor suppressor factors in the mouse granulosa cells. Furthermore, immunofluorescence, RT-qPCR as well as western-blot analysis verified the different expression of related genes in ZEA exposed porcine and mouse granulosa cells. Collectively, these results illustrate the presence of species differences with regards to ZEA effects between porcine and mouse ovarian granulosa cells, in vitro.

• 出版日期2018-7-1
• 单位青岛农业大学; 青岛大学