High-resolution two-dimensional gel electrophoresis analysis of atrial tissue proteome reveals down-regulation of fibulin-1 in atrial fibrillation.

作者：Garcia Angel^*; Eiras Sonia; Parguina Andres F; Alonso Jana; Rosa Isaac; Salgado Somoza Antonio; Rico Trinidad Yolanda; Teijeira Fernandez Elvis; Ramon Gonzalez Juanatey Jose

来源：International Journal of Cardiology, 2011, 150(3): 283-290.

DOI：10.1016/j.ijcard.2010.04.036

• Atrial fibrillation
• Heat shock proteins
• Fibulin-1
• Two-dimensional gel electrophoresis
• Proteomics

Background: Atrial fibrillation (AF) is the most common cardiac arrhythmia found in clinical practice. We combined high-resolution two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) to compare the atrial proteome of subjects with AF versus controls with sinus rhythm (SR). Our aim was to identify novel differentially regulated proteins that could be related to the development of the arrhythmia.
Methods: Human atrial appendage tissue samples from patients undergoing heart surgery with AF or SR were analyzed by high-resolution 2-DE. Proteins of interest were identified by MS and validated by western blotting and inmunohistochemistry.
Results: Our analysis allowed the detection of over 2300 protein spots per gel. Following differential image analysis, we found 22 spot differences between the AF and SR groups in the 4-7 isoelectric point range, leading to the identification of 15 differentially regulated proteins. The main group of proteins identified was that of heat shock proteins (HSPs), including TRAP-1, HspB3, Hsp.6 and AHA1. Some of the differences detected between AF and SR for the above proteins were due to post-translational modifications. In addition, we identified the structural protein fibulin-1 as down-regulated in atrial tissue from AF patients.
Conclusions: High-resolution 2-DE analysis of human atrial tissue revealed that AF is associated with changes in structural proteins and an important number of HSPs. The lower expression of the structural protein fibulin-1 in atrial tissue from AF patients might reflect the myocardial structural changes that take place in the arrhythmia.

• 出版日期2011-8-4