Autophagy regulated by lncRNA HOTAIR contributes to the cisplatin-induced resistance in endometrial cancer cells

作者：Sun Meng Yao; Zhu Jian Yong; Zhang Chun Yan; Zhang Miao; Song Ya Nan; Rahman Khalid; Zhang Li Jun^*; Zhang Hong^*

来源：Biotechnology Letters, 2017, 39(10): 1477-1484.

DOI：10.1007/s10529-017-2392-4

• Autophagy
• Cisplatin-resistance
• Endometrial cancer
• HOTAIR
• Long noncoding RNA

To identify whether lncRNAs (long non-coding RNA) participate in the regulation of cisplatin-resistant induced autophagy in endometrial cancer cells.
Autophagy activity was significantly boosted in cisplatin-resistant Ishikawa cells, a human endometrial cancer cell line, compared with that in parental Ishikawa cells. After analyzing the overall long noncoding RNA (lncRNA) profiling, a meaningful lncRNA, HOTAIR, was identified. It was down-regulated simultaneously in cisplatin-resistant Ishikawa cells and parental Ishikawa cells treated with cisplatin. RNA interference of HOTAIR reduced the proliferation of cisplatin-resistant Ishikawa cells and enhanced the autophagy activity of cisplatin-resistant Ishikawa cells with or without cisplatin treatment, in addition, beclin-1, multidrug resistance (MDR), and P-glycoprotein (P-gp) were mediated by lncRNA HOTAIR.
It is clear that lncRNAs, specifically HOTAIR, can regulate the cisplatin-resistance ability of human endometrial cancer cells through the regulation of autophagy by influencing Beclin-1, MDR, and P-gp expression.

• 出版日期2017-10
• 单位中国人民解放军第二军医大学