科研之友
微信
新浪微博
Facebook
分享链接
摘要
An indirect electrochemical sensing strategy for the determination of ofloxacin (OFL) was developed using cupric ion (Cu2+) as an electrochemical probe. The method was based on the complexation of OFL with Cu2+, which was investigated by UV-visible spectrophotometry and differential pulse voltammetry (DPV). When OFL was added into the electrolyte solution containing Cu2+, the electro-reduction peak current of Cu2+ on glassy carbon electrode (GCE) was decreased. Some influencing factors in terms of pH, quiet time, and reaction time were systematically studied. Under optimal conditions, the Cu2+ reduction peak current difference (Delta I (p)) before and after adding OFL was found to be linear to the concentration of OFL in the range from 1.0 x 10(-7) to 1.0 x 10(-4) M. The detection limit (3S/N) was 8.2 x 10(-8) M. Moreover, the proposed sensor displayed high selectivity and good reproducibility, which was successfully applied to the detection of OFL in pharmaceutical tablet and chicken fodder.
- 出版日期2015-11
- 单位四川轻化工大学; 华中科技大学
