LOVTRAP: an optogenetic system for photoinduced protein dissociation

作者：Wang Hui; Vilela Marco; Winkler Andreas; Tarnawski Miroslaw; Schlichting Ilme; Yumerefendi Hayretin; Kuhlman Brian; Liu Rihe^*; Danuser Gaudenz^*; Hahn Klaus M^*

来源：Nature Methods, 2016, 13(9): 755-758.

DOI：10.1038/NMETH.3926

LOVTRAP is an optogenetic approach for reversible light induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (K-d). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

• 出版日期2016-9
• 单位河北医科大学