Defining the DNA mismatch repair-dependent apoptotic pathway in primary cells: Evidence for p53-independence and involvement of centrosomal caspase 2

作者：Narine Kelly A D; Keuling Angela M; Gombos Randi; Tron Victor A; Andrew Susan E^*; Young Leah C

来源：DNA Repair, 2010, 9(2): 161-168.

DOI：10.1016/j.dnarep.2009.11.010

• DNA mismatch repair
• Msh6
• UVB
• Apoptosis
• p53
• Caspase 2
• Centrosome

Many studies have shown that DNA mismatch repair (MMR) has a role beyond that of repair in response to several types of DNA damage, including ultraviolet radiation (UV). We have demonstrated previously that the MMR-dependent component of UVB-induced apoptosis is integral to the suppression of UVB-induced tumorigenesis. Here we demonstrate that Msh6-dependent UVB-induced apoptotic pathway is both activated via the mitochondria and p53-independent. In addition, we have shown for the first time that caspase 2, an initiator caspase, localizes to the centrosomes in mitotic primary mouse embryonic fibroblasts, irrespective of MMR status and UVB treatment.

• 出版日期2010-2-4