A New Trihydroxylated Fatty Acid and Phytoecdysteroids from Rhizomes of Trillium govanianum

作者：Rahman Shafiq Ur^*; Adhikari Achyut^*; Ismail Muhammad; Shah Muhammad Raza; Khurram Muhammad; Anis Itrat; Ali Farman

来源：Records of Natural Products, 2017, 11(3): 323-327.

• Trillium govanianum
• trilliaceae
• govanic acid
• antifungal
• phytoecdysteroids

A crude hydro-methanolic extract of Trillium govanianum ex D. Don (Melanthiaceae, Trilliaceae) rhizomes and its subsequent solvents soluble fractions were tested against different fungal strains i.e. Trichophyton rubrum ATCC 40051, Aspergillus niger ATCC 16888, Candida albicans ATCC 18804, Microsporum canis ATCC 32903 and Fusarium lini ATCC 16888. The hydro-methanolic extract showed significant activity against T. rubrum and M. canis with 80 and 75% inhibitions respectively. Among the fractions, chloroform soluble fraction showed 90% inhibition, with minimum inhibitory concentration (MIC) of 10 mu g/mL against T. rubrum followed by ethyl acetate, butanol and n-hexane fractions. The bio-activity guided isolation of chloroform soluble fraction leads to a new trihydroxylated fatty acid, named govanic acid (1) along with two known phytoecdysteroids i.e. 20-hydroxyecdysone (2) and 5, 20-dihydroxyecdysone (3). The structures of isolated compounds were elucidated through 1D, 2D-NMR spectroscopic data analysis. All compounds (1-3) in T. govanianum are reported herein for the first time. Compound 1 showed significant activity against T. rubrum with 70% inhibition and MIC value of 5 mu g/mL, but lack of activity against the other test strains.

• 出版日期2017-6