Adsorption properties of an activated carbon for 18 cytokines and HMGB1 from inflammatory model plasma

作者：Inoue Satoru; Kiriyama Kentaro; Hatanaka Yoshihiro; Kanoh Hirofumi^*

来源：Colloids and Surfaces B: Biointerfaces , 2015, 126: 58-62.

DOI：10.1016/j.colsurfb.2014.12.015

• Activated carbon
• Mesopore
• Adsorption
• Cytokine
• Inflammatory model plasma

The ability of an activated carbon (AC) to adsorb 18 different cytokines with molecular weights ranging from 8 kDa to 70 kDa and high mobility group box-1 (HMGB1) from inflammatory model plasma at 310K and the mechanisms of adsorption were examined. Porosity analysis using N-2 gas adsorption at 77 K showed that the AC had micropores with diameters of 1-2 nm and mesopores with diameters of 5-20 nm. All 18 cytokines and HMGB1 were adsorbed on the AC; however, the shapes of the adsorption isotherms changed depending on the molecular weight. The adsorption isotherms for molecules of 8-10 kDa, 10-20 kDa, 20-30 kDa, and higher molecular weights were classified as H-2, L-3, S-3, and S-1 types, respectively. These results suggested that the adsorption mechanism for the cytokines and HMGB1 in the mesopores and on the surface of the AC differed as a function of the molecular weight. On the basis of these results, it can be concluded that AC should be efficient for cytokine adsorption.

• 出版日期2015-2-1