Stable encapsulation of active enzyme by application of multilayer nanofilm coatings to alginate microspheres

作者：Srivastava R; Brown JQ; Zhu HG; McShane MJ^*

来源：Macromolecular Bioscience, 2005, 5(8): 717-727.

DOI：10.1002/mabi.200500061

• alginate
• enzyme immobilization
• layer-by-layer self assembly
• microspheres
• nanofilms

In an effort to improve the stability for long-term biosensor use, layer-by-layer self-assembly was explored as a potential technique to provide a diffusion barrier to encapsulated glucose oxidase inside alginate microspheres (<5 mu m), fabricated using an emulsification technique. The total loss of encapsulated enzyme was reduced to less than 25 and 15% with the application of single PAH/PSS and crosslinked PAH/PAA coatings, respectively, in comparison to at least a 45% loss observed with uncoated and PDDA/PSS-coated microspheres. Furthermore, it was found that enzyme within PAH/PSS- and crosslinked PAH/PAA-coated spheres retained more than 84 and 60% of initial activity, respectively, after three months, whereas uncoated and PDDA/ PSS-coated microspheres retained less than 20%.

• 出版日期2005-8-12