An Effective New Cryopreservation Procedure for Pancreatic Islets Using Hollow Fiber Vitrification

作者：Nagaya M^*; Matsunari H; Kanai T; Maehara M; Nakano K; Umeki I; Katsumata Y; Kasai Y; Sakai R; Kobayashi M; Honda M; Abe N; Watanabe M; Umeyama K; Nagashima H^*

来源：Hormone and Metabolic Research, 2016, 48(8): 540-549.

DOI：10.1055/s-0042-102628

• islet transplantation
• pancreatic islet
• vitrification
• cryopreservation
• hollow fiber

The present study aimed at establishing a new cryopreservation method for mouse pancreatic islets by vitrification using hollow fibers as a container. A unique feature of the hollow fiber vitrification (HFV) method is that this method achieves stable vitrification using a minimum volume of cryoprotectant (CPA) solution, thereby ensuring high viability of the islets. The cytotoxicity, optimum composition, and concentration of the CPAs for vitrifying islets were examined. The viability, functional-integrity of vitrified islets were evaluated in comparison with those vitrified by conventional methods. Insulin secretion was measured in vitro by a static incubation assay and the metabolic functions was tested after transplantation into Streptozotocin-induced diabetic mice. The combination of 15% dimethyl sulfoxide+15% ethylene glycol resulted in the best CPA solution for the HFV of islets. HFV showed the highest viability in comparison to 2 vitrification methods, open pulled straws and vitrification with EDT324 solution. The vitrified islets stably expressed -cells markers NeuroD, Pancreatic and duodenal homeobox-1, and MafA. Transplantation of the vitrified islets achieved euglycemia of the host diabetic mice and response to an intraperitoneal glucose tolerance test to a similar extent as non-vitrified transplanted islets. The HFV method allows for efficient long-term cryopreservation of islets.

• 出版日期2016-8