Deciphering gene expression profiles generated from DNA microarrays and their applications in oral medicine

作者：Kuo WP; Whipple ME; Epstein JB; Jenssen TK; Santos GS; Ohno Machado L; Sonis ST

来源：Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 2004, 97(5): 584-591.

DOI：10.1016/j.tripleo.2003.11.016

Genome-wide monitoring of gene expression profiles using DNA microarrays provides a unique approach to exploring the biological processes underlying oral diseases and disorders by providing a comprehensive survey of a cell's or tissue's transcriptional mapping. This revolutionary technology allows for the simultaneous assessment of the transcription levels of tens of thousands of genes, and of their relative expression between normal and diseased cells. As microarray data analysis evolves, there is a widespread hope that microarrays will significantly impact our ability to explore the genetic changes associated with disease etiology and development, ultimately leading to the discovery of new biomarkers for disease diagnosis and prognosis prediction as well as new therapeutic tools. The goal of this manuscript is to review 2 of the most commonly used microarray technologies, provide an overview of data analyses involved in a typical microarray experiment, and comment upon the application of microarrays to oral medicine.

• 出版日期2004-5