Chromosomal circularization in Streptomyces griseus by nonhomologous recombination of deletion ends

作者：Inoue S; Higashiyama K; Uchida T; Hiratsu K; Kinashi H^*

来源：Bioscience Biotechnology and Biochemistry, 2003, 67(5): 1101-1108.

• Streptomyces
• chromosomal circularization
• chromosomal deletion
• genome fluidity
• nonhomologous recombination

Streptomyces linear chromosomes frequently cause deletions at both ends spontaneously or by various mutagenic treatments, and concomitantly display dynamic structural changes such as circularization and arm replacement. We have cloned and sequenced the fusion junctions of circularized chromosomes in two deletion mutants of Streptomyces griseus. No homology and a 1-bp overlap were found between the deletion ends of the mutant chromosomes. Taking this together with previous results, we concluded that chromosomal circularization in Streptomyces occurs by nonhomologous recombination between deletion ends.

• 出版日期2003-5