A divalent metal-dependent self-cleaving DNAzyme with a tyrosine side chain

作者：Lam Curtis H; Hipolito Christopher J; Hollenstein Marcel; Perrin David M^*

来源：Organic and Biomolecular Chemistry, 2011, 9(20): 6949-6954.

DOI：10.1039/c1ob05359k

The enzymatic incorporation of a phenol-modified 2'-deoxyuridine triphosphate gave rise to a modified DNA library that was subsequently used in an in vitro selection for ribophosphodiester-cleaving DNAzymes in the presence of divalent zinc and magnesium cations. After 11 rounds of selection, cloning and sequencing resulted in 14 distinct sequences, the most active of which was Dz11-17PheO. Dz11-17PheO self-cleaved an embedded ribocytidine with an observed rate constant of 0.20 +/- 0.02 min(-1) in the presence of 10 mM Mg(2+) and 1 mM Zn(2+) at room temperature. The activity was inhibited at low concentrations of Hg(2+) cations and somewhat higher concentrations of Eu(3+) cations.

• 出版日期2011