Arsenate induces the expression of fungal genes involved in As transport in arbuscular mycorrhiza

作者：Gonzalez Chavez Ma del Carmen A; del Pilar Ortega Larrocea Maria; Carrillo Gonzalez Rogelio; Lopez Meyer Melina; Xoconostle Cazares Beatriz; Gomez Susana K; Harrison Maria J; Miguel Figueroa Lopez Alejandro; Maldonado Mendoza Ignacio E^*

来源：Fungal Biology, 2011, 115(12): 1197-1209.

DOI：10.1016/j.funbio.2011.08.005

• Arsenate exclusion
• Arbuscular mycorrhizal fungus tolerance to Arsenic
• GiArsA gene
• High-affinity Pi/As transporter

We utilized the two-compartment system to study the effect of arsenic (As) on the expression of the Glomus intraradices high-affinity phosphate transporter GiPT, and the GiArsA gene, a novel protein with a possible putative role as part of an arsenite efflux pump and similar to ArsA ATPase. Our results show that induction of GiPT expression correlates with As(V) uptake in the extra-radical mycelium of G. intraradices. We showed a time-concerted induction of transcript levels first of GiPT, followed by GiArsA, as well as the location of gene expression using laser microdissection of these two genes not only in the extra-radical mycelium but also in arbuscules. This work represents the first report showing the dissection of the molecular players involved in arbuscular mycorrhizal fungus (AMF)-mediated As tolerance in plants, and suggests that tolerance mediated by AMP may be caused by an As exclusion mechanism, where fungal structures such as the extra-radical mycelium and arbuscules may be playing an important role. Our results extend knowledge of the mechanisms underlying As efflux in arbuscular mycorrhizal fungi and mechanisms related to As tolerance.

• 出版日期2011-12