Development of germ cell neoplasia in situ in chinchilla rabbits

作者：Maria Vigueras Villasenor Rosa; Montelongo Solis Paola; Chavez Saldana Margarita; Gutierrez Perez Oscar; Cortes Trujillo Lucero; Cesar Rojas Castaneda Julio

来源：Histology and Histopathology, 2016, 31(5): 573-584.

• Germ cell neoplasia in situ
• POU5F1
• c-kit
• Rabbit
• Animal model

The present study was designed to describe the development of germ cell neoplasia in situ in Chinchilla rabbit by administration of estradiol. The study was performed in rabbits distributed into two groups: control and 17 beta-estradiol. The determination of histological alterations and POU5F1 and c-kit proteins employed as biomarkers for the diagnosis of this neoplasia was carried out. Testicular descent and complete spermatogenesis were observed in the control group. The protein biomarkers were negative. However, in the rabbits treated with estradiol, the testes remained undescended with the gonocytes undifferentiated to spermatogonia. There were histological lesions owing to germ cell neoplasia in situ and positive to POU5F1 and c-kit proteins. These findings indicate that the chinchilla rabbit is an ideal model to study this neoplasia in which the histological characteristics and biomarkers of the disease could be clearly observed. Using this model we suggested that the persisting gonocytes could be responsible for the development of germ cell neoplasia in situ.

• 出版日期2016-5