Adaptive optics enables three-dimensional single particle tracking at the sub-millisecond scale

作者：Juette Manuel F; Rivera Molina Felix E; Toomre Derek K; Bewersdorf Joerg^*

来源：Applied Physics Letters, 2013, 102(17): 173702.

DOI：10.1063/1.4803538

We present the integration of an adaptive optics element into a feedback-driven single particle tracking microscope. Our instrument captures three-dimensional (3D) trajectories with down to 130 mu s temporal resolution for dynamic studies on the nanoscale. Our 3D beam steering approach tracks particles over an axial range of >6 mu m with similar to 2 ms mechanical response times and isolates the sample from any tracking motion. Tracking of transport vesicles containing Alexa488-labeled transferrin glycoprotein in living cells demonstrates the speed and sensitivity of our instrument.

• 出版日期2013-4-29