Diterpenoids and triterpenoids in hairy roots of Salvia sclarea

作者：Kuzma L^*; Skrzypek Z; Wysokinska H

来源：Plant Cell, Tissue and Organ Culture, 2006, 84(2): 171-179.

DOI：10.1007/s11240-005-9018-6

• aethiopinone
• Agrobacterium rhizogenes
• ferruginol
• hydroxy derivatives of ursolic acid
• 1-oxoaethiopinone
• salvipisone
• transformation

Hairy root culture of Salvia sclarea L. was established following infection with Agrobacterium rhizogenes LBA 9402. The culture was grown in growth regulator-free half-strength B5 Gamborg medium with 30 g l(-1) sucrose and was investigated with respect to its capability of producing diterpenoids and triterpenoids. Four diterpenoids (ferruginol, salvipisone, aethiopinone and l-oxoaethiopinone) and two ursene-type triterpenoids (2 alpha,3 alpha-dihydroxy-urs-12-en-28-oic acid and 2 alpha,3 alpha,24-trihydroxy-urs-12-en-28-oic acid) were isolated from the hairy roots. The presence of three sterols (beta-sitosterol, stigmasterol and campesterol), as well as oleanolic and ursolic acids was also shown by GC-MS analysis. The quantitative and qualitative differences in diterpenoid and triterpenoid production patterns between hairy roots grown in the light and in the dark were described.

• 出版日期2006-2