Detection of inhibitors of Candida albicans Cdr transporters using a diS-C-3(3) fluorescence

作者：Szczepaniak Joanna; Lukaszewicz Marcin; Krasowska Anna^*

来源：Frontiers in Microbiology, 2015, 6.

DOI：10.3389/fmicb.2015.00176

• Candida albicans
• ABC transporters
• inhibitors
• diS-C-3(3)
• enniatin A
• beauvericin

Candida albicans is a major cause of opportunistic and life threatening, systemic fungal infections. Hence new antifungal agents, as well as new methods to treat fungal infections, are still needed. The application of inhibitors of drug-efflux pumps may increase the susceptibility of C. albicans to drugs. We developed a new fluorescence method that allows the in vivo activity evaluation of compounds inhibiting of C. albicans transporters. We show that the potentiometric dye 3,3'-dipropylthiacarbocyanine iodide diS-C-3(3) is pumped out by both Cdr1 and Cdr2 transporters. The fluorescence labeling with diS-C-3(3) enables a real-time observation of the activity of C. albicans Cdr1 and Cdr2 transporters. We demonstrate that enniatin A and beauvericin show different specificities toward these transporters. Enniatin A inhibits diS-C-3(3) efflux by Cdr1 while beauvericin inhibits both Cdr1p and Cdr2p.

• 出版日期2015-3-9