A journey through PROTEOSONICS

作者:Araujo J E; Oliveira E; Kouvonen P; Corthals G L; Lodeiro C; Santos H M*; Capelo J L
来源:Talanta, 2014, 121: 71-80.
DOI:10.1016/j.talanta.2013.12.054

摘要

Ultrasonic energy is gaining momentum in Proteomics. It helps to shorten many proteomics workflows in an easy and efficient manner. Ultrasonic energy is nowadays used for protein extraction, solubilisation and cell disruption, to speed protein identification, protein quantification, peptide profiling, metal-protein complexes characterisation and imaging mass spectrometry. The present review gives a perspective of the latest achievements in ultrasonic-based sample treatment for proteomics as well as provides the basic concepts and the tools of the trade to efficiently implement this tool in proteomics labs.

  • 出版日期2014-4