SOE-LRed: A simple and time-efficient method to localize genes with point mutations onto the Escherichia coli chromosome

作者：Benson Ryan W; Cafarelli Tiziana M; Godoy Veronica G^*

来源：Journal of Microbiological Methods, 2011, 84(3): 479-481.

DOI：10.1016/j.mimet.2010.12.020

• SOE PCR
• Transformation
• E. coli
• dinB
• Lambda Red
• Point mutation

We use a powerful method to replace wild-type genes on the chromosome of Escherichia coli. Using a unique form of PCR, we generate easily constructible gene fusions bearing single point mutations. Used in conjunction with homologous recombination, this method eliminates cloning procedures previously used for this purpose.

• 出版日期2011-3
• 单位东北大学