Substrate-Dependent Functional Alterations of Seven CYP2C9 Variants Found in Japanese Subjects

作者：Maekawa Keiko^*; Harakawa Noriko; Sugiyama Emiko; Tohkin Masahiro; Kim Su Ryang; Kaniwa Nahoko; Katori Noriko; Hasegawa Ryuichi; Yasuda Kazuki; Kamide Kei; Miyata Toshiyuki; Saito Yoshiro; Sawada Jun ichi

来源：Drug Metabolism and Disposition, 2009, 37(9): 1895-1903.

DOI：10.1124/dmd.109.027003

CYP2C9 is a polymorphic enzyme that metabolizes a number of clinically important drugs. In this study, catalytic activities of seven alleles found in Japanese individuals, CYP2C9*3 (I359L), *13 (L90P), *26 (T130R), *28 (Q214L), *30 (A477T), *33 (R132Q), and *34 (R335Q), were assessed using three substrates ( diclofenac, losartan, and glimepiride). When expressed in a baculovirus-insect cell system, the holo and total (apo and holo) CYP2C9 protein expression levels were similar among the wild type (CYP2C9.1) and six variants except for CYP2C9.13. A large part of CYP2C9.13 was present in the apo form P420. Compared with CYP2C9.1, all variants except for CYP2C9.34 exhibited substrate-dependent changes in K(m), V(max), and intrinsic clearance (V(max/)K(m)). For diclofenac 4'-hydroxylation, the intrinsic clearance was decreased markedly ( by > 80%) in CYP2C9.13, CYP2C9.30, and CYP2C9.33 and variably (63-76%) in CYP2C9.3, CYP2C9.26, and CYP2C9.28 due to increased K(m) and/or decreased V(max) values. For losartan oxidation, CYP2C9.13 and CYP2C9.28 showed 2.5- and 1.8-fold higher K(m) values, respectively, and all variants except for CYP2C9.34 showed > 77% lower Vmax and intrinsic clearance values. For glimepiride hydroxylation, the Km of CYP2C9.13 was increased 7-fold, and the V(max) values of all variants significantly decreased, resulting in reductions in the intrinsic clearance by > 80% in CYP2C9.3, CYP2C9.13, CYP2C9.26, and CYP2C9.33 and by 56 to 75% in CYP2C9.28 and CYP2C9.30. These findings suggest the necessity for careful administration of losartan and glimepiride to patients bearing these six alleles.

• 出版日期2009-9