NUCLEOTIDE-SEQUENCES OF 2 GENOMIC DNAS ENCODING PEROXIDASE OF ARABIDOPSIS-THALIANA

作者：INTAPRUK C; HIGASHIMURA N; YAMAMOTO K; OKADA N; SHINMYO A; TAKANO M

来源：Gene, 1991, 98(2): 237-241.

DOI：10.1016/0378-1119(91)90179-F

• RECOMBINANT DNA
• HORSERADISH ENZYMES
• SEQUENCE HOMOLOGIES
• EXONS
• INTRONS
• ISOZYME

The peroxidase (EC 1.11.1.7)-encoding gene of Arabidopsis thaliana was screened from a genomic library using a cDNA encoding a neutral isozyme of horseradish, Armoracia rusticana, peroxidase (HRP) as a probe, and two positive clones were isolated. From the comparison with the sequences of the HRP-encoding genes, we concluded that two clones contained peroxidase-encoding genes, and they were named prxCa and prxEa. Both genes consisted of four exons and three introns; the introns had consensus nucleotides, GT and AG, at the 5' and 3' ends, respectively. The lengths of each putative exon of the prxEa gene were the same as those of the HRP-basic-isozyme-encoding gene, prxC3, and coded for 349 amino acids (aa) with a sequence homology of 89% to that encoded by prxC3. The prxCa gene was very close to the HRP-neutral-isozyme-encoding gene, prxClb, and coded for 354 aa with 91% homology to that encoded by prxClb. The aa sequence homology was 64% between the two peroxidases encoded by prxCa and prxEa.

• 出版日期1991-2-15