Toll-like receptors 2 and 3 enhance melanogenesis and melanosome transport in human melanocytes

作者：Koike Saaya; Yamasaki Kenshi^*; Yamauchi Takeshi; Inoue Mai; Shimada Ohmori Ryoko; Tsuchiyama Kenichiro; Aiba Setsuya

来源：Pigment Cell & Melanoma Research, 2018, 31(5): 570-584.

DOI：10.1111/pcmr.12703

• melanogenesis
• melanosome
• Rab27A
• toll-like receptor
• ultraviolet

Because little is known about how the innate immune response influences skin-pigmentation, we examined whether Toll-like receptor (TLR) agonists participate in melanogenesis and melanosome transportation. We observed that TLR2/2 agonist HKLM and TLR3 agonist Poly(I:C) increased the amount of extracellular melanin from primary human epidermal melanocytes. HKLM, but not Poly(I:C), increased the melanogenic genes such as tyrosinase and dopachrome tautomerase. Poly(I:C) increased the expression of Rab27A, a molecule that facilitates melanosome transport to perimembranous actin filament. UVB irradiation induced Rab27A and melanosome transportation in a similar manner of Poly(I:C). SiRNA for TLR3 or Rab27A suppressed the perimembranous accumulation of Gp100-positive vesicles in melanocytes and decreased melanin transfer to neighboring keratinocytes induced by both Poly(I:C) and UVB. These results suggest that the microenvironment in the epidermis and innate immune stimuli, such as microbiome and ultraviolet represented here by TLR2 and TLR3 agonists, could affect the melanogenesis in human melanocytes.

• 出版日期2018-9