Development of a model of tissue with potentially malignant disorders (PMD) in the hamster cheek pouch to explore the long-term potential therapeutic and/or toxic effects of different therapeutic modalities

作者：Heber Elisa M; Monti Hughes Andrea; Pozzi Emiliano C C; Itoiz Maria E; Aromando Romina F; Molinari Ana J; Garabalino Marcela A; Nigg David W; Trivillin Veronica A; Schwint Amanda E^*

来源：Archives of Oral Biology, 2010, 55(1): 46-51.

DOI：10.1016/j.archoralbio.2009.10.010

• Potentially malignant disorders
• PMD
• Hamster cheek pouch
• Carcinogenesis protocols
• Long-term follow-up

Objective: Given that locoregional recurrences developing from a tissue with potentially malignant disorders (PMD) in oral mucosa are a frequent cause of therapeutic failure, and that tissue with PMD is dose-limiting, the aim of the present study was to develop a model of tissue with PMD to evaluate the long-term therapeutic/toxic effects of different therapeutic modalities.
Materials and methods: We evaluated 5 carcinogenesis protocols based on topical application of the carcinogen dimethyl-1,2-benzanthracene in the hamster cheek pouch, twice a week for 4, 6, 7, and 8 weeks and the classical 3 times a week for 12 weeks.
Results: Long-term follow-up (8 months after protocol completion) was only possible with the 4- and 6-week carcinogenesis protocols. Tumour development increased progressively with time and aggressiveness of the carcinogenesis protocols. The time at which tumours developed in >90% of the animals was at protocol completion (TO) for the 12-week protocol, 1 month post-TO for the 8-week protocol, 3 months post-TO for the 7-week protocol and 4 months post-TO for the 6-week protocol. <40% of the animals in the 4-week protocol developed tumours within the 8 months follow-up period. DNA synthesis rose as a function of time and protocol aggressiveness.
Conclusions: The 6-week carcinogenesis protocol was selected for long-term studies of different therapeutic modalities in tissue with PMD because it permitted long-term follow-up and guaranteed tumour development in >90% of the animals.

• 出版日期2010-1