Antibacterial efficacy and cytotoxicity studies of copper (II) and titanium (IV) substituted hydroxyapatite nanoparticles

作者：Li Yan; Ho Jiahui; Ooi Chui Ping^*

来源：Materials Science & Engineering C-Materials for Biological Applications, 2010, 30(8): 1137-1144.

DOI：10.1016/j.msec.2010.06.011

• Ion exchange reaction
• Hydroxyapatite
• Copper
• Titanium
• Antibacterial effect
• Cytotoxicity

Cu(2+) and Ti(4+) substituted hydroxyapatite (CuHA; TiHA) in different molar ratios (M(added)/Ca(added) = 0.01, 0.05, 0.10 and 0.15) were synthesized using wet chemical method coupled with ion exchange reaction. All characterization results confirmed the samples were hydroxyapatite (HA). XRD data showed that all samples were single phased. Both intensity and width of XRD peaks did not change significantly as substitution concentration increased. The FTIR spectra revealed the occurrence of CO(3)(2-) ions (B-type carbonated HA). ICP data indicated that the content of Cu and Ti in CuHA and TiHA increased with initial metal salt concentration. Visual examination by FESEM showed all samples possessed elongated spheroid morphology with crystal size of around 70 nm. The CuHA nanoparticles possessed high antibacterial property against Escherichia coli. This was probably due to the leaching of Cu(2+) ions which subsequently led to the lysis of bacteria. TiHA had slight antibacterial effect compared to pure HA at high Ti content (Ti(added)/Ca(added) = 0.1). Agar diffusion and extraction cytotoxicity assay revealed that the presence of Cu(2+) was cytotoxic while Ti(4+) was only slightly toxic to osteoblasts.

• 出版日期2010-10-12
• 单位南阳理工学院