Automated microfluidic sample-preparation platform for high-throughput structural investigation of proteins by small-angle X-ray scattering

作者：Lafleur Josiane P; Snakenborg Detlef; Nielsen Soren S; Moller Magda; Toft Katrine N; Menzel Andreas; Jacobsen Jes K; Vestergaard Bente; Arleth Lise; Kutter Jorg P^*

来源：Journal of Applied Crystallography, 2011, 44: 1090-1099.

DOI：10.1107/S0021889811030068

A new microfluidic sample-preparation system is presented for the structural investigation of proteins using small-angle X-ray scattering (SAXS) at synchrotrons. The system includes hardware and software features for precise fluidic control, sample mixing by diffusion, automated X-ray exposure control, UV absorbance measurements and automated data analysis. As little as 15 mu l of sample is required to perform a complete analysis cycle, including sample mixing, SAXS measurement, continuous UV absorbance measurements, and cleaning of the channels and X-ray cell with buffer. The complete analysis cycle can be performed in less than 3 min. Bovine serum albumin was used as a model protein to characterize the mixing efficiency and sample consumption of the system. The N2 fragment of an adaptor protein (p120-RasGAP) was used to demonstrate how the device can be used to survey the structural space of a protein by screening a wide set of conditions using high-throughput techniques.

• 出版日期2011-10