Dietary Plasma Proteins Modulate the Adaptive Immune Response in Mice with Acute Lung Inflammation

作者：Maijo Monica; Miro Lluisa; Polo Javier; Campbell Joy; Russell Louis; Crenshaw Joe; Weaver Eric; Moreto Miquel; Perez Bosque Anna^*

来源：Journal of Nutrition, 2012, 142(2): 264-270.

DOI：10.3945/jn.111.149070

We examined the effects of oral plasma protein supplements on the pulmonary adaptive immune response in mice challenged with intranasal LPS. C57BL/6 mice were fed a control diet or a diet supplemented with plasma proteins [spray-dried plasma (SDP) 80 g/kg] or with an Ig concentrate [(IC) 20 g/kg] from postnatal d 19 (weaning) until d 34. Mice were challenged with PBS or [PS from Escherichia coliat d 33 and killed 24 h later for leukocyte analyses or at d 34 and killed 6 h later for cytokine determination. LPS induced the activation of T helper (Th) lymphocytes in lung and blood and this response was reduced by SDP and IC (P %26lt; 0.05). In both tissues, LPS increased the Th1 and Th2 subpopulations and this effect was inhibited by the two plasma protein supplements (P %26lt; 0.05). The LPS challenge increased the expression of all the cytokines studied (P %26lt; 0.01). SDP and IC reduced the expression of IFN gamma, IL-5, IL-12p40, IL-12p70, IL-13, and IL-17 in both tissues, whereas they increased the percentage of regulatory Th lymphocytes in lung, even in PBS-treated mice (P %26lt; 0.05). LPS reduced the concentration of mature TGF beta 1 (P %26lt; 0.05) in the lung but did not modify the expression of IL-10. Mice exposed to LPS and supplemented with SDP or IC showed an increased expression of the anti-inflammatory cytokine IL-10 (P %26lt; 0.05). Moreover, the two supplements increased the concentration of IL-10 in intestinal mucosa (P %26lt; 0.05). Our results show that plasma supplementation reduces the immune response that characterizes the acute lung inflammation syndrome. J. Nutr. 142: 264-270, 2012.

• 出版日期2012-2