Hemodynamics associated with atrial fibrillation directly alters thrombotic potential of endothelial cells

作者:Simmers Michael B; Cole Banumathi K; Ogletree Martin L*; Chen Zhu; Xu Yiming; Kong Ling jie; Mackman Nigel; Blackman Brett R; Wamhoff Brian R*
来源:Thrombosis Research, 2016, 143: 34-39.
DOI:10.1016/j.thromres.2016.04.022

摘要

An experimental in vitro model of the hemodynamics that occur in atrial fibrillation (AFib) in the left atrial appendage (LAA) was developed to study changes in human endothelial cell thrombotic potential. We applied human-derived sinus rhythm and AFib hemodynamic shear stress patterns to primary human endothelial cells (ECs) in culture. We found that ECs exposed to AFib hemodynamics have increased thrombotic potential as measured by increased expression of pro-thrombotic gene markers and fibrin deposition on the endothelium. Treatment with the factor Xa inhibitor, apixaban, attenuated fibrin deposition thickness while increasing fibrin density at the endothelial cell surface. This study suggests that altered hemodynamics associated with AFib play a key role in driving the thrombotic potential of the LAA endothelium.

  • 出版日期2016-7