A Combining Signal Amplification of Atom Transfer Radical Polymerization and Redox Polymerization for Visual Biomolecules Detection

作者：Zhuang, Dequan; Shen, Heyun; Liu, Guodong; Yu, Changyuan; Yang, Jing^*

来源：Journal of Polymer Science Part A: Polymer Chemistry , 2014, 52(19): 2791-2799.

DOI：10.1002/pola.27303

• ATRP
• atom transfer radical polymerization
• branched
• molecular diagnosis
• redox polymerization
• redox polymers
• SA
• signal amplification

A novel combination of atom transfer radical polymerization (ATRP) and redox polymerization is here used to allow instrument-free visualization of special biomolecules for which dynamic polymer growth is used in signal amplification. In this method, the convenient and mild redox polymerization-assisted amplification with cerium ammonium (IV) nitrate as oxidant at the second stage was achieved by directly using the hydroxyl groups from poly(hydroxyethyl methacrylate) (PHEMA) synthesized via ATRP at the first stage. The brushed polymers poly(hydroxylethyl methacrylate)-branched-poly (acrylamide) (PHEMA-branched-PAM) prepared by successive ATRP and redox polymerization in situ drastically grew up at the detected biomolecules spot to improve the visibility of biomolecule and simplify the detection procedure. With the proposed strategy, the signal amplification of streptavidin (SA) as model detected biomolecule was investigated on two different substrates such as silicon wafer and gold, respectively. As a result, detection limit of SA was demonstrated on the gold substrates where binding of 1.0 ng/mL SA was differentiable from the background using ellipsometry. Moreover, binding of 0.5 nmol/L DNA led to visually distinguishable spots on the gold surface under mild condition. The proposed method exhibited an efficient amplification performance for molecules detection, and paved a new way for visual diagnosis of biomolecules.

• 出版日期2014-10-1
• 单位北京化工大学