Autotrophic denitrification by nitrate-dependent Fe(II) oxidation in a continuous up-flow biofilter

作者:Zhou, Jun; Wang, Hongyu*; Yang, Kai*; Ji, Bin; Chen, Dan; Zhang, Huining; Sun, Yuchong; Tian, Jun
来源:Bioprocess and Biosystems Engineering, 2016, 39(2): 277-284.
DOI:10.1007/s00449-015-1511-7

摘要

A continuous-upflow biofilter packed with sponge iron was constructed for nitrate removal under an anaerobic atmosphere. Microbacterium sp. W5, a nitrate reducing and Fe(II) oxidizing strain, was added to the biofilter as an inoculum. The best results were achieved when NO3 (-)-N concentration was 30 mg/L and Fe2+ was 800 mg/L. Nitrite in influent would inhibit nitrate removal and aqueous Fe2+ resulted in encrustation. Fe(II)EDTA would prevent cells from encrustation and the maximum nitrogen removal efficiency was about 90 % with Fe(II)EDTA level of 1100 mg/L. Nitrate reduction followed first-order reaction kinetics. Characteristics of biofilms were analyzed by X-ray fluorescence spectroscopy.