Association of angiotensin type 2 receptor gene polymorphisms with ureteropelvic junction obstruction in Brazilian patients

作者：Miranda Debora M; Dos Santos Augusto Cesar Jr; Sarubi Helena C; Bastos Rodrigues Luciana; Rosa Daniela Valadao; Freitas Izabella S; De Marco Luiz Armando; Oliveira Eduardo A; Simoes e Silva Ana Cristina

来源：Nephrology, 2014, 19(11): 714-720.

DOI：10.1111/nep.12308

• angiotensin type 2 receptor gene
• congenital anomalies of the kidney and urinary tract
• renin angiotensin system
• ureteropelvic junction obstruction

AimThe angiotensin type 2 (AT(2)) receptor takes part in the process of ureteric bud during kidney development. Therefore, the gene encoding AT(2) receptor, the AGTR2 gene located in the X chromosome, is a potential candidate for genetic association with Congenital Anomalies of the Kidney and Urinary Tract (CAKUT). This study aimed to investigate whether AGTR2 gene polymorphisms are associated with CAKUT in general or even with common phenotypes of CAKUT in a Brazilian sample of paediatric patients. %26lt;br%26gt;MethodsWe analyzed 290 paediatric patients with CAKUT and 262 healthy controls from the same geographic area. TaqMan single-nucleotide polymorphism (SNP) genotyping assays for AGTR2 gene at rs1403543, rs3736556, rs35474657, rs5193 and rs5194 were performed. The sample was in Hardy-Weinberg Equilibrium for all five SNPs. %26lt;br%26gt;ResultsThe presence of CAKUT in general was not significantly associated with the SNPs included in this study. However, when patients were segregated according to major phenotypes, the diagnosis of Ureteropelvic Junction Obstruction (UPJO) was significantly associated with AGTR2 gene polymorphisms at rs3736556 and at rs5194. On the other hand, the diagnoses of vesicoureteral reflux and of multicystic dysplastic kidney were not associated with AGTR2 gene polymorphisms. %26lt;br%26gt;ConclusionOur results support that the AGTR2 gene may contribute to the pathogenesis of UPJO and the genetic origin of CAKUT could vary according to phenotype expression. %26lt;br%26gt;Summary at a Glance This authors examine the AGTR2 gene polymorphisms in Brazilian children with CAKUT, and find an association of AGTR2 gene polymorphisms with ureteropelvic junction obstruction. Since CAKUT is a prevalent disease in children with CKD, the findings observed in this manuscript will contribute to a better understanding of the pathogenesis of CAKUT.

• 出版日期2014-11