Analgesic, Anti-inflammatory, Anti-lipoxygenase Activity and Characterization of Three Bioactive Compounds in the Most Active Fraction of Leptadenia reticulata (Retz.) Wight & Arn. - A Valuable Medicinal Plant

作者：Mohanty Sudipta Kumar^*; Swamy Mallappa Kumara; Middha Sushil Kumar; Prakash Lokesh; Subbanarashiman Balasubramanya; Maniyam Anuradha

来源：Iranian Journal of Pharmaceutical Research, 2015, 14(3): 933-942.

• Leptadenia reticulate
• Anti-inflammatory activity
• Analgesic activity
• Lipid peroxidation inhibition
• Pro-inflammatory cytokines

Leptadenia reticulata was reported to be used for several medicinal purposes. The present study was undertaken to evaluate anti-inflammatory, analgesic and lipid peroxidation inhibition activities of L. reticulata. The anti-inflammatory assay was performed by lambda-carrageenan and formalin induced paw edema test. Pro inflammatory mediators (IL2, IL6, TNF-alpha) in serum of treated and control organism were analyzed by quantitative ELISA. Lipid peroxidation inhibition was measured by thiobarbituric acid reactive substances (TBARS) assay. Analysis of the most active fraction revealed the presence of one phenolic compound (p-coumaric acid), two flavonoids (rutin and quercetin) which also determined quantitatively. The ethyl acetate fraction at 600 mg/Kg significantly inhibited lambda-carrageenan and formalin induced paw edema by 60.59% and 59.24% respectively. Notable reduction in percentage of writhing (76.25%), induced by acetic acid signifies the potent analgesic activity. Lower level of pro-inflammatory cytokines (IL-2, IL-6, TNF-alpha) in serum at the 4th hour of lambda-Carrageenan injection indicated the inhibition of cyclooxigenase-2 (Cox-2), Nitric oxide (NO) and release of prostaglandin to prevent inflammation. The study also demonstrated the decrease in malonaldehyde (MDA) concentration which revealed the lipid peroxidation inhibition potential of the plant. Our finding provides evidence for potent biological activities in tested model which is supported by its characterized bioactive compounds and ethnomedicinal relevance.

• 出版日期2015