A fluorescence assay that detects long branches in the starch polysaccharide amylopectin

作者:Beeren Sophie R*; Hindsgaul Ole
来源:Chemical Communications, 2014, 50(13): 1530-1532.
DOI:10.1039/c3cc48163h

摘要

Long alpha(1-4)-linked glucopyranose branches in the starch polysaccharide amylopectin can be detected by the specific binding of an anionic amphiphilic fluorescent probe. The probe forms spermidine-stabilised micelles in water resulting in fluorescence quenching. By extracting the probe from the micelles polysaccharides are detected in a "turn-on'' fluorescence assay.

  • 出版日期2014