ADAM10 regulates proliferation, invasion, and chemoresistance of bladder cancer cells

作者：Fu, Lin; Liu, Nan; Han, Yong; Xie, Chengyao; Li, Qingchang^*; Wang, Enhua

来源：Tumor Biology, 2014, 35(9): 9263-9268.

DOI：10.1007/s13277-014-2201-9

• Bladder cancer
• Proliferation
• Invasion
• Migration
• Chemoresistance

A disintegrin and metalloprotease 10 (ADAM10) is upregulated in several cancers and associates with malignant cancer progression. However, its expression pattern in bladder cancer remains unexplored. In the present study, we examined ADAM10 expression in 105 bladder cancer specimens using immunohistochemistry. We found ADAM10 overexpression in 51 of 105 (48.5 %) bladder cancer specimens. ADAM10 overexpression associated with advanced tumor stage (p = 0.001) and tumor grade (p = 0.018). To explore its biological functions in bladder cancer cells, small interfering RNA (siRNA) knockdown was performed in 5,637 and T24 cell lines. Cell Counting Kit-8 (CCK8) assay and Matrigel invasion assay showed that ADAM10 depletion decreased cell proliferation, migration, and invasion. In addition, ADAM10 knockdown increased the level of cisplatin-induced apoptosis. In conclusion, ADAM10 is overexpressed in bladder cancer and regulates malignant cell growth and invasion, which makes it a candidate therapeutic target.

• 出版日期2014-9
• 单位中国医科大学