Comparative Cytogenetics Among Populations of Hollandichthys multifasciatus (Teleostei: Characidae)

作者：Balen Rafael Ernesto; Noleto Rafael Bueno^*; Vicari Marcelo Ricardo; Artoni Roberto Ferreira; Cestari Marta Margarete

来源：Zoological Science, 2013, 30(2): 105-109.

DOI：10.2108/zsj.30.105

• characids
• neotropical region
• karyotype
• fluorochromes
• rDNA

Two populations of Hollandichthys multifasciatus from headwaters of adjacent river coastal basins in Parana state, southern Brazil, were cytogenetically studied in order to evaluate intraspecific divergences. Both populations presented 2n = 50 chromosomes, divided into 14m + 18sm + 18st in the sample from Antonina and 14m + 20sm + 16st for the population from Guaraquecaba. Analyses of active nucleolar organizer regions (Ag-NORs) and fluorescent in situ hybridization (FISH) with 18S rDNA probes revealed a single metacentric pair bearing marks at proximal positions on the short arms. The location of the 5S rDNA and GC-rich sites showed chromosomal divergence between both populations. Therefore, several population markers were detected, reflecting a population differentiation, possibly driven by the formation of Paranagua Bay and Serra do Mar mountain range. Since the existence of a species complex in H. multifasciatus has been previously characterized by morphological, molecular, and karyotype population differentiation, this species needs to be taxonomically studied in detail.

• 出版日期2013-2