Stability of coagulation protein activities in single units or pools of cryoprecipitate during storage at 20-24 degrees C for up to 24 h

作者：Sheffield W P^*; Bhakta V; Jenkins C

来源：Vox Sanguinis, 2016, 110(1): 12-19.

DOI：10.1111/vox.12309

• blood component production
• blood components
• blood processing
• plasma derivatives

Background and Objective Cryoprecipitate is a concentrated source of fibrinogen and other plasma proteins. Cryoprecipitate must be transfused within 4-6 h of thawing and storage at 20-24 degrees C. We compared plasma protein activities in single or pooled cryoprecipitate units stored at 20-24 degrees C for 0, 4 or 24 h.
Materials and Methods Individual cryoprecipitate units (n = 36) were thawed, diluted with sterile saline and sampled over time. Cryoprecipitate pools of eight individual units were assembled either by serial passage of diluent (Method A, n = 6 pools) or by separate dilution into a single collection bag (Method B, n = 6 pools). Fibrinogen, factor VIII, factor XIII and von Willebrand factor activities were measured.
Results No significant losses in activities were found relative to at-thaw values after either 4 or 24 h of storage of individual cryoprecipitate units at 20-24 degrees C; 35 of 36 units contained > 150 mg of fibrinogen. No significant differences were found between activities in single vs. pooled units of cryoprecipitate assembled using either method, or between cryoprecipitate pools made by Method A (80160 ml volume) or Method B (160-240 ml volume) at 0, 4 or 24 h post-thaw; freezing and thawing of pools did not lead to significant activity losses.
Conclusion The stability of fibrinogen and other factors in thawed cryoprecipitate stored at 20-24 degrees C suggests that the shelf life may be safely extended to 24 h provided that sterility is maintained.

• 出版日期2016-1