A novel approach based on low-field NMR for the detection of the pathological components of sputum in cystic fibrosis patients

作者：Abrami Michela; Ascenzioni Fiorentina; Di Domenico Enea Gino; Maschio Massimo; Ventura Alessandro; Confalonieri Marco; Di Gioia Sante; Conese Massimo; Dapas Barbara; Grassi Gabriele^*; Grassi Mario

来源：Magnetic Resonance in Medicine, 2018, 79(4): 2323-2331.

DOI：10.1002/mrm.26876

• mucus
• lung disease
• monitoring
• low-field NMR
• sputum

PurposeDevelopment of a reliable, simple method to monitor lung condition in cystic fibrosis (CF) patients. Lung functionality assessment in CF patients is relevant, as most of them still die of respiratory failure. In lung mucus (sputum) of CF patients, components such as proteins, biopolymers, DNA, bacteria, and mucin are pathologically increased. As lung functionality is related to the amount of the pathological components in the sputum, their determination can help clinicians in monitoring lung condition and planning therapy.
MethodsLow-field NMR was used to evaluate the variation of the relaxation time (T-2m) of the water hydrogens present in CF sputum in relation to the amounts of the pathological components. Low-field NMR was tested in artificial samples (mucin or alginates), then in conditional sputum (saliva from healthy volunteers, added by different amounts of the pathological components), and finally in 12 patients' sputums, in which T-2m was correlated to a commonly used lung monitoring test (i.e., forced expiratory volume in the first second).
ResultsT(2m) significantly (P<0.05) differed between samples with and without pathological components and between healthy and CF patients (P<0.05), in which T-2m correlated (r=0.87) with FEV1.
ConclusionsThe presented method can potentially become a valuable lung-monitoring tool in CF patients. Magn Reson Med 79:2323-2331, 2018.

• 出版日期2018-4